A simple method for measuring organotypic tissue slice culture thickness
نویسندگان
چکیده
منابع مشابه
Simple Method For Stationary Organotypic Slice Culture of Hypothalamic Suprachiasmatic Nucleus
Early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as socalled organotypic brain slice cultures. The present study describes a simple method for stationary long-term organotypic culture for the hypothalamic suprachiasmatic nucleus (SCN). Hypothalamic brain slices from ...
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Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium. They were placed on a sterile, transparent and porous membrane and kept in petri dishes in an incubator. No plasma clot or roller drum were used. This method yields thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypi...
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Organotypic slicing of brain tissue from young rodents has been used as a powerful model system for biomedical research [1], [2], [3]. Organotypic slicing complements cell culture and in vivo studies in multiple facets. This system can be useful for investigating manipulation of cellular signaling pathways without the hindrance of the blood-brain barrier while sacrificing fewer animals in the p...
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Mouse genetics offers a powerful tool determining the role of specific genes during development. Analyzing the resulting phenotypes by immunohistochemical and molecular methods provides information of potential target genes and signaling pathways. To further elucidate specific regulatory mechanisms requires a system allowing the manipulation of only a small number of cells of a specific tissue ...
متن کاملOrganotypic slice culture of the hypothalamic paraventricular nucleus of rat
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain ...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2011
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2011.03.027